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HPLC and mass spectrometric analyses of culture filtrates from an orf15::apr mutant. (A) HPLC elution profile with detection at 311 nm. 2-Hydroxymethyl clavam (2-HMC) and clavam 2-carboxylate (C-2-C) are two of the 5S clavams produced by S. clavuligerus; NAG-clavam is the new 5S clavam found in orf15 and orf16 mutants. (B) Mass spectrum of the NAG-clavam peak at 18.6 min.
