TABLE 4.
Effect of gene disruption mutations on clavulanic acid production
| Mutant gene (similaritya) | Reduction in clavulanic acid productionb (% relative to wild type) in indicated medium
|
|
|---|---|---|
| Soy | SA | |
| orf11 (ferredoxin) | 70-80 | 25-65 |
| orf12 (β-lactamase) | 100 | 100 |
| orf13 (efflux pump) | 75-95 | 75-95 |
| orf14 (acetyltransferase) | 99.5 | 100 |
| orf15 (peptide binding protein) | 100 | 100 |
| orf16 (hypothetical protein) | 100 | 100 |
| orf17 (carboxylase) | 100 | 100 |
| orf18 (PBP) | Not done | Not done |
| orf19 (PBP) | 0 | 0 |
a
Protein with highest level of similarity to that encoded by indicated ORF.
b
Representative mutants were cultured in duplicate and growth experiments were repeated on two or more occasions in two different locations. Typical clavulanic acid production levels for the wild type were 225 μg/ml in Soy medium and 20 μg/ml in SA medium. The limit of detection for clavulanic acid was about 1 μg/ml in Soy medium grown cultures.