FIG. 2.
pH-dependent interaction of acetic acid with FLZ. (A) YNB was adjusted to pH 4.2, without supplement (diamonds), with acetic acid (20 mM; triangles), or with lactic acid (20 mM; squares). FLZ at 64 μg/ml was added to some cultures (closed symbols). (B) YNB was buffered with 165 mM MOPS, pH 7.4. Cultures are represented as for panel A. (C) RPMI 1640, pH 7, cultures are represented as described for panel A. An overnight culture of C. albicans ATCC 32354 was inoculated into each medium to a final cell density of ∼2 × 105 cells/ml. Viability was determined as described in the legend for Fig. 1.