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Membrane disruption assay by using liposomes that encapsulate fluorescent dyes. The leakage of dyes caused by peptide addition was measured with a fluorescence spectrophotometer. At 400 and 1,000 s after the start of measurement, the liposome suspension was spiked with either mastoparan or synthetic poly(arginyl-histidine) at a final concentration of 10 μM and detergents, respectively.
