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. 2011 Mar 10;2(3):e127. doi: 10.1038/cddis.2011.10

Figure 1.

Figure 1

NPR-A small-interfering RNA (siRNA) induces efficient knockdown of NPR-A in murine ES cells. (a) RT-PCR analysis of ES cells transfected with control siRNA or NPR-A siRNAs, showing knockdown of the NPR-A gene 48 h after siRNA transfection. GAPDH was used as the internal control. (b) Real-time PCR analysis of ES cells treated with control siRNA or NPR-A siRNA2. (c) Western blot analysis of ES cells treated as described in panel a, showing a reduced level of the NPR-A protein 48 h after siRNA transfection. β-Actin was used as a loading control. Data represent mean±S.D. (n=3); *P<0.05 (two-tailed t-test)