Figure 4.

Subcellular localization of the lacZ reporter gene in Nes-R26^Met and R26^{LacZ−stop−Met} mice. (a and b) Colocalization studies of X-Gal activity with NeuN protein showing the reporter activity in neuronal cell types. (c and d) Enlarged view of the hippocampus area. (e–h) Colocalization studies of X-Gal activity with GFAP protein showing that the lacZ reporter gene is not predominantly expressed in astrocytes. Panels (g and h) correspond to an enlarged view of the hippocampus. (i–k) Mixed cell cultures derived from E12.5 R26^{LacZ−stop−Met} spinal cords were immunostained for neurofilament-160 (red) and β-galactosidase (green) proteins. (i–n) Astrocyte cultures from P2 R26^{LacZ−stop−Met} spinal cords immunostained for GFAP (red) and β-galactosidase (green). DAPI was used to counterstain nuclei (blue), scale bar: 20 μm