Figure 1.
Analysis of the expression levels of caspase-8 and -10 isoforms. (a) Schematic representation of caspase-10 and -8 protein isoforms. Regions encoded by alternative exons are indicated. DED, death effector domain. (b) Whole cell extracts from various cell lines were loaded on a 15% SDS-polyacrylamide gel as described,40 and analysed by immunoblotting for the expression of caspase-8 and -10 isoforms. The three types of NB cells (S, substrate adherent; I, intermediate; N, neuroblastic) are represented. n.d., not determined. The immunoblot for endogenous caspase-10 detection were revealed using the ECL Advanced western blotting substrate for stronger signal detection. (c) Caspase-8 and -10 mRNA expression levels in tumour cells were compared by semi-quantitative real-time PCR. Plots represent means of three separate experiments