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. 2011 Apr 19;104(10):1575–1586. doi: 10.1038/bjc.2011.133

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Copyright © 2011 Cancer Research UK

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Silencing GLI1 expression impairs motility of SUM149 and rSUM149 cells. Cells were grown to confluence in six-well plates and scratched with a sterile pipette tip. Closure of the wound was monitored over 24 h. In vitro wound-healing assay on SUM149 (A) and rSUM149 cells (B) treated with vehicle (lipofectamine alone), control siRNA, Maob siRNA and GLI1 siRNA. Images were taken at × 40 magnification over 24 h. Wound-healing assay for MCF-7 is shown as a control. Immunoblots are shown to confirm significant knockdown of GLI1 protein only in cells treated with siRNA to GLI1. (C) Images from (A and B) were analysed using CellProfiler software to calculate the area occupied by cells at 0 and 24 h. The percentage of wound occupied was calculated by dividing the non-recovered area at 24 h by the initial wound area at 0 h and subtracting this value as a percent from 100%.