Figure 7.

Bag-1 expression in normal epidermal keratinocytes, epidermal dysplasia and SCC cell lines. Western blot comparing Bag-1, Hsp70, Hsc70 and p53 expression of normal human epidermal keratinocytes (NHK1; passage 3; two independent sample preparations from the same patient are shown) with two epidermal dysplasia cell lines (PM1 and PM3) and five epidermal SCC cell lines (MET2, MET4, SCC-12, SCC-13 and BICR19). All cell lines were grown on the same culture medium for at least 3 days before harvest (see Materials and methods). α-Tubulin was used as a loading control. Asterisks (^*) indicate non-specific antibody bands.