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. 2011 Apr 12;104(9):1500–1504. doi: 10.1038/bjc.2011.113

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Copyright © 2011 Cancer Research UK

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Representative results of the stage 2 PCR using methylated (M) and unmethylated (U) primers for the CDKN2A gene. Lanes 1–20, cervical cytological samples taken from 20 women who were smokers. The presence of a 150 bp product using the methylated primers indicates that the cytological sample was positive for CDKN2A methylation (samples 1, 2, 6, 9, 12, and 16). CpGenome Universal Unmethylated DNA (vial B) (UCB) was used as a positive control for unmethylated DNA. CpGenome Universal Methylated DNA (MC) was used as a positive control for methylated DNA. H₂0a, water from both stages of the nested methylation-specific PCR. H₂0, water from the stage 2 PCR only.