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Fraction purity. A, Coomassie-stained gel of NE and microsome fractions analyzed. B, Western blot of the above fractions stained with organelle markers. ER markers calreticulin and calnexin were absent from NEs, whereas NE markers lamin B2 and the NET LAP2β were absent from microsomes. Similar amounts of total protein were loaded. MM, microsomal membranes.
