Fig. 8.

Screen for PBMC NETs that promote chromatin compaction. HeLa cells stably expressing H2B-GFP were transfected with the same set of nine NETs used in Fig. 7. Only IAG2 displayed strong chromatin condensation, whereas all other NETs (METTL7A, C17orf32, and C20orf3 are shown) exhibited no differences in chromatin compaction or distribution compared with adjacent untransfected cells. It is noteworthy that IAG2 had no effect in the lacO screen and that NETs STT3A and TAPBPL, which altered lacO positioning, had no effect on chromatin compaction.