Skip to main content
. 2011 Mar 29;30(9):1804–1817. doi: 10.1038/emboj.2011.93

Figure 1.

Figure 1

Pelota, Hbs1 and ABCE1 strongly stimulate 48S complex formation from 80S ribosomes by dissociating them into subunits. (A, E) Toe-printing analysis of the influence of different combinations of Pelota, Hbs1, eRF1/eRF3 and ABCE1 on 48S complex formation at 1 mM free Mg2+ on (CAA)nGUS mRNA from 40S subunits or 80S ribosomes. Lanes C, T, A and G depict cDNA sequences corresponding to (CAA)nGUS mRNA. The positions of toe-prints corresponding to ribosomal complexes are indicated. (B) Purified recombinant Pelota and Hbs1 resolved by SDS–PAGE. (C) Thin-layer chromatography analysis of the GTPase activity of Hbs1. (D) Dissociation of vacant 80S ribosomes containing [32P]60S subunits by different combinations of Pelota, Hbs1, ABCE1, eRFs and eIF6 at 1.5 mM free Mg2+, assayed by SDG centrifugation.