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. 2011 Mar 29;30(9):1804–1817. doi: 10.1038/emboj.2011.93

Figure 3.

Figure 3

Pelota, Hbs1 and ABCE1 cannot dissociate pre-termination and elongation complexes assembled on MVHL-STOP mRNA containing an ∼400-nt-long 3′-UTR. (A) Structure of MVHL-STOP mRNA. (B–E) Investigation by (B, C) SDG centrifugation and (D, E) toe-printing analysis of the influence of Pelota, Hbs1 and ABCE1 at 1.5 mM free Mg2+ on (B, D) pre-TCs and (C, E) ECs containing MV-peptidyl-tRNA assembled on MHVL-STOP mRNA. (B, C) Pre-TCs and ECs were assembled using [32P]60S subunits. Upper fractions were omitted for clarity. (D, E) Lanes C, T, A and G depict cDNA sequence corresponding to MVHL-STOP mRNA; the positions of toe-prints corresponding to ribosomal complexes are indicated.