Figure 4.

Pelota, Hbs1 and ABCE1 can dissociate pre-termination and elongation complexes assembled on MVHL-STOP mRNA after their treatment with RelE. (A) Primer-extension analysis of A-site mRNA cleavage induced by RelE in 80S initiation and in pre- and post-translocation ECs containing MV-peptidyl-tRNA that were assembled on MVHL-STOP mRNA. (B–D) Dissociation by Pelota, Hbs1 and ABCE1 at 1.5 mM free Mg²⁺ of (B, C) post- and pre-translocation ECs containing MV-peptidyl-tRNA and (D) pre-TCs assembled on MVHL-STOP mRNA using [³²P]60S subunits depending on their treatment with RelE, assayed by SDG centrifugation. Upper fractions were omitted for clarity.