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Kinetics and Mg2+ dependence of dissociation by Pelota, Hbs1 and ABCE1 of pre-termination complexes containing 3 mRNA nucleotides downstream of the P-site. Ribosomal association of [35S-Cys]MVHC-peptidyl tRNA after incubation of pre-TCs assembled on MVHC-3 mRNA with Pelota, Hbs1 and ABCE1 (A) at different Mg2+ concentrations, and (B) for different time periods at 1.5 mM Mg2+, assayed by SDG centrifugation. Upper fractions were omitted for clarity.
