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Ectopic expression of TSC22D11073aa overrides OIS. (A) Cell proliferation assay of HDF (Tig3(et)-16i) transduced with a vector driving expressing TSC22D1.1 or TSC22D1.2a in the presence or absence of BRAFE600, as indicated. Cells were fixed and stained 10 days after transduction with BRAFE600. (B) In parallel to (A), samples were collected for immunoblotting to determine TSC22D1.1 levels. β-Actin serves as loading control.
