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. 2011 Mar 29;30(9):1753–1765. doi: 10.1038/emboj.2011.95

Figure 7.

Figure 7

TSC22D1 regulation of OIS involves its dimerization partner THG1. (A) HDF (Tig3(et)-16i) were transduced with lentivirus expressing the indicated genes or shRNAs. THG1 and BRAF levels were determined by immunoblotting 10 days after transduction with BRAF. β-Actin serves as loading control. (B) HDF were transduced with BRAFE600-expression vector or control vector. Eight days after transduction, cells were harvested, lysed and subjected to immunoprecipitation (IP) with THG1-specific antibody. Immunoprecipitates were resolved and immunoblotting was performed with TSC22-specific antibody detecting all TSC family members including THG1 (open triangle). An antibody of the same species and immunoglobulin serve as controls. HC indicates the heavy chain, LC indicates the light chain of immunoglobulins. The image showing the input control was exposed for 2 min, the IP blot for 20 s. (C) Cell proliferation assay of HDF (Tig3(et)-16i) transduced with the indicated constructs. Cells were fixed and stained 10 days after transduction with BRAFE600. (D) In parallel to (C), cells were harvested and analysed for THG1 protein levels by immunoblotting.