Table 1. Primer sequences used for amplification of TGFBI.
| Exon | Primer name | Primer sequence | Melting temperature | Product size (bp) |
|---|---|---|---|---|
| 4 |
TGFBI-4F |
cctcgtcctctccacctgta |
60 |
328 |
| |
TGFBI-4R |
tcggggaagtaaggcagttc |
61 |
|
| 5 |
TGFBI-5F |
gtctgcagcccctaactgac |
60 |
317 |
| |
TGFBI-5R |
cacaaagagggtgggttgtc |
60 |
|
| 6 |
TGFBI-6F |
gcttgtggaacccacatttt |
60 |
392 |
| |
TGFBI-6R |
tcaggggaacctgctctatg |
60 |
|
| 7 |
TGFBI-7F |
tgggtttggcttctgttttc |
60 |
376 |
| |
TGFBI-7R |
catggcaggtggtatgttca |
60 |
|
| 8 |
TGFBI-8F |
gggtcctcatctgagagaacag |
60 |
480 |
| |
TGFBI-8R |
gtcacaacccacacatttgc |
60 |
|
| 9 |
TGFBI-9F |
cgagatgacattcctgctga |
60 |
369 |
| |
TGFBI-9R |
ttttggttgagctgagtgga |
59 |
|
| 10 |
TGFBI-10F |
tccaaactcaaggagggatg |
60 |
365 |
| |
TGFBI-10R |
tcagcaaccagttctcatgc |
60 |
|
| 11 |
TGFBI-11F |
tgaccctgctacatgctctg |
60 |
374 |
| |
TGFBI-11R |
ccatcccaagtctggaaggt |
61 |
|
| 12 |
TGFBI-12F |
cctctcagcgtggtgaggta |
61 |
261 |
| |
TGFBI-12R |
ggccctgagggatcactact |
60 |
|
| 13 |
TGFBI-13F |
gggcagggagttcttcattt |
60 |
351 |
| |
TGFBI-13R |
gctgcaacttgaaggttgtg |
60 |
|
| 14 |
TGFBI-14F |
ctgggcgacaagattgaaac |
61 |
433 |
| |
TGFBI-14R |
tgtggtgcattcaaaaccaa |
61 |
|
| 15 |
TGFBI-15F |
ggaaatgtgagccagaaagc |
60 |
284 |
| |
TGFBI-15R |
agcagccaaggaagacagg |
60 |
|
| 16 |
TGFBI-16F |
accttccccttcctcttcct |
60 |
281 |
| |
TGFBI-16R |
caaaggccaggcttctttta |
60 |
|
| 17 |
TGFBI-17F |
ttggccctggtccttgag |
62 |
250 |
| TGFBI-17R | gcggcccatgtacattaaa | 59 |