TABLE 1.

Antimicrobial activities of DA-7867 compared with those of other antibiotics against aerobic and anaerobic bacteria

Organism(s) (no. of isolates tested) and antimicrobial agent	MIC (μg/ml)			% of isolatesd
	Range	50% of strains	90% of strains	S	I	R
Methicillin-susceptible Staphylococcus aureus (33)
DA-7867	0.12-0.25	0.25	0.25	100	NAc	NA
Linezolid	1-2	2	2	100	NA	NA
Erythromycin	0.12-128	0.25	64	79	0	21
Clindamycin	≤0.06->128	≤0.06	≤0.06	97	0	3
Cotrimoxazole	≤0.06-16	≤0.06	0.12	97	NA	3
Gentamicin	≤0.06-128	≤0.06	64	79	3	18
Levofloxacin	0.12-1	0.25	0.25	100	0	0
Tetracycline	0.12-128	0.25	32	79	0	21
MRSA (30)
DA-7867	0.06-0.25	0.12	0.25	100	NA	NA
Linezolid	1-2	2	2	100	NA	NA
Erythromycin	0.5->128	>128	>128	7	0	93
Clindamycin	≤0.06->128	>128	>128	10	0	90
Cotrimoxazole	≤0.06-128	0.12	64	83	NA	17
Gentamicin	0.25->128	128	>128	7	0	93
Levofloxacin	0.5->128	>128	>128	7	0	93
Tetracycline	0.5-128	64	128	13	0	87
Methicillin-susceptible coagulase-negative staphylococci (22)
DA-7867	0.06-0.25	0.12	0.12	100	NA	NA
Linezolid	0.5-2	1	1	100	NA	NA
Erythromycin	≤0.06-128	0.12	32	77	0	23
Clindamycin	≤0.06->128	≤0.06	0.12	96	0	4
Cotrimoxazole	≤0.06-8	0.12	8	82	NA	18
Gentamicin	≤0.06-64	1	4	96	0	4
Levofloxacin	0.06-8	0.25	1	91	0	9
Tetracycline	≤0.06-128	0.25	64	64	5	32
Methicillin-resistant coagulase-negative staphylococci (29)
DA-7867	≤0.03-0.25	0.12	0.25	100	NA	NA
Linezolid	0.25-2	1	2	100	NA	NA
Erythromycin	0.25->128	>128	>128	14	0	86
Clindamycin	≤0.06->128	>128	>128	45	3	52
Cotrimoxazole	≤0.06-8	2	8	55	NA	45
Gentamicin	≤0.06-128	32	128	24	14	62
Levofloxacin	0.12-32	0.5	8	52	10	38
Tetracycline	0.25->128	2	64	62	3	35
Vancomycin-susceptible Enterococcus faecalis (49)
DA-7867	≤0.06-0.12	0.12	0.12	100	0	0
Linezolid	0.5-2	1	2	100	0	0
Ampicillin	0.25-4	0.5	2	100	NA	0
Erythromycin	≤0.12->128	>128	>128	12	8	80
Levofloxacin	1-64	2	64	61	0	39
Tetracycline	0.5->128	64	128	12	4	84
Vancomycin	1-2	2	2	100	0	0
Teicoplanin	≤0.12-1	0.25	0.5	100	0	0
Vancomycin-resistant E. faecalis (10)
DA-7867	≤0.06-0.12	≤0.06	0.12	100	0	0
Linezolid	0.5-2	1	1	100	0	0
Ampicillin	0.5-4	2	4	100	NA	0
Erythromycin	>128	>128	>128	0	0	100
Levofloxacin	16-64	64	64	0	0	100
Tetracycline	16-64	32	64	0	0	100
Vancomycin	>128	>128	>128	0	0	100
Teicoplanin	64->128	>128	>128	0	0	100
Vancomycin-susceptible E. faecium (29)
DA-7867	≤0.06-0.12	0.12	0.12	100	0	0
Linezolid	0.5-2	2	2	100	0	0
Ampicillin	1->128	>128	>128	3	NA	97
Erythromycin	≤0.12->128	>128	>128	3	7	90
Levofloxacin	1-128	64	128	7	0	93
Tetracycline	≤0.12-64	0.5	8	90	7	3
Vancomycin	0.5-4	0.5	1	100	0	0
Teicoplanin	0.25-1	0.5	1	100	0	0
Vancomycin-resistant E. faecium (30)
DA-7867	≤0.06-0.12	0.12	0.12	100	0	0
Linezolid	0.5-2	2	2	100	0	0
Ampicillin	16->128	>128	>128	0	NA	100
Erythromycin	64->128	>128	>128	0	0	100
Levofloxacin	8-128	64	128	0	0	100
Tetracycline	≤0.12-32	0.25	1	90	0	10
Vancomycin	>128	>128	>128	0	0	100
Teicoplanin	4->128	64	128	3	13	84
Streptococcus pneumoniae (22)
DA-7867	≤0.008-0.12	0.03	0.03	100	NA	NA
Linezolid	0.5-2	1	1	100	NA	NA
Penicillin G	0.03-2	1	2	14	63	23
Cefotaxime	≤0.008-2	1	2	36	50	14
Clindamycin	≤0.06->128	>128	>128	27	0	73
Erythromycin	0.12->128	128	128	5	0	95
Cotrimoxazole	0.5-64	8	32	36	0	64
Levofloxacin	8-128	64	128	0	0	100
Tetracycline	8-64	16	32	0	0	100
Streptococcus pyogenes (15)
DA-7867	≤0.008-0.12	0.06	0.12	100	NA	NA
Linezolid	0.25-1	0.5	1	100	NA	NA
Penicillin G	≤0.008-0.015	≤0.008	≤0.008	100	NA	NA
Cefotaxime	≤0.008-0.015	≤0.008	0.015	100	0	0
Clindamycin	≤0.06	≤0.06	≤0.06	100	0	0
Erythromycin	0.12->128	0.12	16	87	0	13
Levofloxacin	0.12-2	0.5	1	100	0	0
Streptococcus agalactiae (15)
DA-7867	≤0.008-0.12	0.06	0.12	100	NA	NA
Linezolid	0.015-1	0.5	1	100	NA	NA
Penicillin G	0.015-0.06	0.03	0.06	100	NA	NA
Cefotaxime	≤0.008-0.06	0.06	0.06	100	0	0
Clindamycin	≤0.06->128	≤0.06	128	73	0	27
Erythromycin	≤0.06->128	≤0.06	>128	87	0	13
Levofloxacin	0.25-0.5	0.5	0.5	100	0	0
Moraxella catarrhalis (24)
DA-7867	0.25-8	0.5	0.5	96	NA	NA
Linezolid	2-32	4	4	96	NA	NA
Penicillin G	0.015-32	8	16	9	NA	91
Cefaclor	0.25-16	1	8	96	4	0
Clindamycin	≤0.06-32	0.5	2	70	25	4
Erythromycin	≤0.06-64	0.12	0.5	91	0	9
Cotrimoxazole	0.12-8	0.25	1	96	NA	4
Levofloxacin	0.015-0.25	0.06	0.06	100	0	0
Tetracycline	≤0.06-16	0.25	0.5	91	0	9
Gentamicin	≤0.06-0.25	0.12	0.25	100	0	0
Haemophilus influenzae (24)
DA-7867	0.25-4	2	2	NA	NA	NA
Linezolid	2-8	8	8	NA	NA	NA
Ampicillin	0.25->128	32	>128	21	0	79
Ampicillin-sulbactam	0.25-8	2	8	58	NA	42
Cefaclor	1-64	8	32	50	29	21
Cefuroxime	0.25-32	1	8	84	8	8
Cefotaxime	≤0.008-1	0.03	0.06	100	NA	NA
Azithromycin	0.5-4	2	4	100	NA	NA
Cotrimoxazole	0.25-32	16	32	17	4	79
Levofloxacin	0.03-1	0.03	0.06	100	NA	NA
Tetracycline	0.12-8	0.5	8	71	4	25
Peptostreptococcus spp. (56)
DA-7867	≤0.06-2	≤0.06	0.12	NA	NA	NA
Linezolid	0.5-8	1	2	NA	NA	NA
Ampicillin	≤0.06-32	0.25	16	75	0	25
Ampicillin-sulbactam	≤0.06-16	0.25	16	89	11	0
Piperacillin	≤0.06-32	0.25	16	100	0	0
Piperacillin-tazobactam	≤0.06-32	0.12	16	100	0	0
Cefoxitin	≤0.06-16	0.5	8	100	0	0
Cefotetan	≤0.06-128	1	64	77	4	20
Imipenem	≤0.06-2	≤0.06	2	100	0	0
Clindamycin	≤0.06->128	0.5	64	75	4	21
Metronidazole	0.25-32	2	16	84	14	2
Chloramphenicol	1-64	2	4	95	0	5
Clostridium perfringens (17)
DA-7867	0.12	0.12	0.12	NA	NA	NA
Linezolid	2	2	2	NA	NA	NA
Ampicillin	≤0.06-0.12	≤0.06	0.12	100	0	0
Ampicillin-sulbactam	≤0.06-0.12	≤0.06	0.12	100	0	0
Piperacillin	≤0.06-0.5	0.25	0.5	100	0	0
Piperacillin-tazobactam	≤0.06-1	0.25	0.5	100	0	0
Cefoxitin	0.12-2	1	2	100	0	0
Cefotetan	≤0.06-2	1	2	100	0	0
Imipenem	≤0.06-0.12	≤0.06	0.12	100	0	0
Clindamycin	≤0.06-4	2	4	53	47	0
Metronidazole	4-16	16	16	41	59	0
Chloramphenicol	2-4	4	4	100	0	0
Vancomycin	0.5-1	0.5	1	NA	NA	NA
Clostridium difficile (15)
DA-7867	≤0.06-0.25	0.12	0.25	NA	NA	NA
Linezolid	0.5-4	2	2	NA	NA	NA
Ampicillin	0.5-2	0.5	1	73	20	7
Ampicillin-sulbactam	0.5-2	0.5	1	100	0	0
Piperacillin	2-8	2	8	100	0	0
Piperacillin-tazobactam	2-8	2	8	100	0	0
Cefoxitin	128->128	128	128	0	0	100
Cefotetan	16-32	32	32	27	73	0
Imipenem	4-16	8	8	13	80	7
Clindamycin	1->128	>128	>128	7	7	87
Metronidazole	1-4	4	4	100	0	0
Chloramphenicol	2-32	8	32	33	27	40
Vancomycin	0.25-2	0.5	1	NA	NA	NA
Other Clostridium spp. (10)
DA-7867	≤0.06-1	0.12	0.5	NA	NA	NA
Linezolid	0.5-8	2	8	NA	NA	NA
Ampicillin	0.12->128	0.5	32	50	20	30
Ampicillin-sulbactam	0.12-16	0.25	1	90	10	0
Piperacillin	0.5->256	1	32	90	0	10
Piperacillin-tazobactam	0.5-16	2	16	100	0	0
Cefoxitin	0.5-128	8	64	50	30	20
Cefotetan	0.5->128	16	>128	70	10	20
Imipenem	≤0.06-2	0.5	2	100	0	0
Clindamycin	≤0.06->128	2	>128	50	0	50
Metronidazole	1->128	32	64	30	10	60
Chloramphenicol	0.5-16	4	8	90	10	0
Vancomycin	0.5->16	2	16	NA	NA	NA
Other anaerobic gram-positive rodsa (14)
DA-7867	≤0.06	≤0.06	≤0.06	NA	NA	NA
Linezolid	0.25-1	1	1	NA	NA	NA
Ampicillin	≤0.06-4	0.25	4	71	7	21
Ampicillin-sulbactam	≤0.06-4	0.25	4	100	0	0
Piperacillin	≤0.06-32	2	16	100	0	0
Piperacillin-tazobactam	≤0.06-32	0.5	16	100	0	0
Cefoxitin	≤0.06-128	4	32	79	14	7
Cefotetan	0.5-128	4	64	57	21	21
Imipenem	≤0.06-2	≤0.06	1	100	0	0
Clindamycin	≤0.06->128	≤0.06	>128	79	0	21
Metronidazole	0.5->128	32	>128	36	7	57
Chloramphenicol	0.5-4	2	4	100	0	0
Vancomycin	0.5-1	1	1	NA	NA	NA
Bacteroides fragilis (34)
DA-7867	1-2	1	2	NA	NA	NA
Linezolid	4	4	4	NA	NA	NA
Ampicillin-sulbactam	0.5-16	1	8	94	6	0
Piperacillin	4->256	32	>256	59	3	38
Piperacillin-tazobactam	0.06-8	2	4	100	0	0
Cefoxitin	4->128	8	32	88	3	9
Cefotetan	2->128	8	128	82	0	18
Imipenem	≤0.06-2	0.25	0.5	100	0	0
Clindamycin	0.5->128	>128	>128	35	6	59
Metronidazole	2-8	4	8	100	0	0
Chloramphenicol	4-8	4	8	100	0	0
B. thetaiotaomicron (15)
DA-7867	1-8	2	4	NA	NA	NA
Linezolid	4-8	4	8	NA	NA	NA
Ampicillin-sulbactam	1-64	2	32	80	7	13
Piperacillin	16->256	64	>256	27	33	40
Piperacillin-tazobactam	4->256	16	16	93	0	7
Cefoxitin	8-64	16	32	53	40	7
Cefotetan	8->128	128	>128	7	20	73
Imipenem	0.12-16	0.5	4	93	0	7
Clindamycin	4->128	>128	>128	0	20	80
Metronidazole	2-8	2	4	100	0	0
Chloramphenicol	4-8	8	8	100	0	0
Other Bacteroides spp. (11)
DA-7867	0.5-4	2	2	NA	NA	NA
Linezolid	2-4	4	4	NA	NA	NA
Ampicillin-sulbactam	0.5-32	8	16	64	27	9
Piperacillin	8->256	>256	>256	27	9	64
Piperacillin-tazobactam	2-128	4	128	82	0	18
Cefoxitin	4-64	16	32	64	27	9
Cefotetan	16->128	32	128	18	36	45
Imipenem	0.25-2	0.5	1	100	0	0
Clindamycin	≤0.12->128	>128	>128	27	0	73
Metronidazole	1-4	4	4	100	0	0
Chloramphenicol	4-8	8	8	100	0	0
Other anaerobic gram-negative rodsb (27)
DA-7867	≤0.06-2	0.25	1	NA	NA	NA
Linezolid	0.5-8	2	8	NA	NA	NA
Ampicillin-sulbactam	≤0.06-4	1	4	100	0	0
Piperacillin	0.06->256	16	128	67	22	11
Piperacillin-tazobactam	≤0.06-16	≤0.06	4	100	0	0
Cefoxitin	≤0.06-16	1	8	100	0	0
Cefotetan	≤0.06-64	4	32	67	30	4
Imipenem	≤0.06-2	≤0.06	2	100	0	0
Clindamycin	≤0.06->128	≤0.06	>128	70	4	26
Metronidazole	≤0.12-8	2	8	100	0	0
Chloramphenicol	1-8	2	4	100	0	0

^aFour Eubacterium spp., three Bifidobacterium spp., four Actinomyces spp., and three Propionibacterium acnes strains.

^bNineteen Prevotella and Porphyromonas spp. and eight Fusobacterium spp.

^cNA, not applicable.

^dS, susceptible; I, intermediate; R, resistant.