Table 1. List of primers used for real time PCR analyses.
| GENE | FOR PRIMER | REW PRIMER | AMPLICON |
| GnRH3 | TTAGCATGGAGTGGAAAGGAAGGTTG | CTTTCAGAGGCAAACCTTCAGCAT | 255 |
| Kiss1 | ACAGACACTCGTCCCACAGATG | CAATCGTGTGAGCATGTCCTG | 131 |
| Kiss2 | ATTCTCTTCATGTCTGCAATGGTCA | TGCTTTCTCAGGTAAAGCATCATTG | 154 |
| LH | AATGCCTGGTGTTTCAGACC | AGTATGCGGGGAAATCCTCT | 288 |
| TGFβ1 | TCGCTTTGTCTCCAAGGACT | TGCAAGAGAGTTGCCATTTG | 234 |
| BMP15 | AGGGTGACCGGATCACTATG | TGCTGCCAGACTTTTTAGACC | 291 |
| LHR | CATTAACCTGCCCGACTGTT | AGTATGCGGGGAAATCCTCT | 164 |
| mPRα | CGGTTGTGATGGAGCAGATT | AGTAGCGCCAGTTCTGGTCA | 185 |
| mPRβ | CAACGAGCTGCTGAATGTGT | GGGCCAGTTCAGAGTGAGAC | 197 |
| ACT | AAAGTCCTCGCTGGAGACAA | TGCGAGCTGTGTGTGTATGA | 242 |
Primers sequences were designed using Primer3 (v. 0.4.0) software (Accession numbers: GNRH3: NM_182887.2; KISS1: NM_001113489.1; KISS2: NM_001142585.1; LH: NM_205622.2; TGFβ1: NM_182873; BMP15: NM_001020484; LHR NM_205625.1; MPRα: NM_183345; MPRβ: NM_183344; ACT: NM_001168286.1).