Table 1. Details of PCRs performed.

PCR	Primers and probes	Reagents	Conditions
gag R1gag R2	419F 1154R [3]gagIF gagIR [1]	Invitrogen Platinum Taq	4 min at 94°C(1 min 94°C, 1 min 57°C, 1 min 72°C)×45 cycles and 10 min 72°C.
gag R1gag R2	419F 1154R [3]gagIF gagIR [1]	Applied Biosystems Taq Gold LD	10 min at 95°C(1 min 95°C, 1 min 57°C, 1 min 72°C)×45 cycles and 10 min 72°C.
XMRV Taq Man	XMRV Probe, F, R [17]	Qiagen Quanti Tect Probe kit	15 min at 95°C(15 secs 95°C, 1 min 60°C)×60 cycles.
XMRV/pMLV Taq Man	P2, F3, R4	Qiagen Quanti Tect Probe kit	15 min at 95°C(15 secs 95°C, 1 min 60°C)×60 cycles.
XMRV/pMLV with IAPE Taq Man	P2, F3, R4IAPE D1Probe, D1F, D1R	Invitrogen Platinum Taq	4 min at 95°C(15 secs 95°C, 1 min 72°C)×60 cycles.
CDC pol	XPOLOF XPOLOR [20] XPOLIF XPOLIR [20]	Invitrogen Platinum Taq	4 min at 94°C(30 secs 94°C, 30 secs 57°C, 1 min 72°C)×45 cycles and 10 min 72°C.

400 nM concentrations of primers and 200 nM probes were used in all the TaqMan assays.