Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May 25;6(5):e20152. doi: 10.1371/journal.pone.0020152

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Ullal et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Phosphorylation of Rtt107 upon DNA damage is dependent on the ATR kinase, Mec1. A DSB was induced at the MAT locus in a wildtype strain expressing Ddc2-GFP (CCG6983) and Ddc1-GFP in a mec1Δ mutant (CCG7143) both also expressing Rtt107-9myc. Cells were collected at indicated times after HO induction and scored for formation of Ddc2 and Ddc1 foci. The binding of Rtt107 around the MAT DSB locus on chromosome III was assayed by chromatin immunoprecipitation at the indicated time points, after (cut- 2 hours) and before (uncut) DSB induction. The averages of two independent experiments with the corresponding standard deviation are shown. A locus on chromosome VI was used as a control. (B) Rtt107 enrichment to regions flanking the HO-induced DSB is dependent on the function of the Smc5–Smc6 complex. A DSB was induced at the MAT locus in wildtype (CCG6983) and smc6–9 (CCG6985) strains expressing Ddc2-GFP and Rtt107-9myc tagged at permissive (25°C) temperature. The binding of Rtt107 around the MAT DSB locus on chromosome III was assayed by chromatin immunoprecipitation at the indicated time points, after (cut- 2 hours) and before (uncut) DSB induction in wildtype and smc6–9 cells at 25°C. The averages of two independent experiments with corresponding standard deviations are shown. A locus on chromosome VI was used as a control. (C) Rtt107 enrichment to regions flanking the HO-induced DSB is dependent on the function of the Smc5–Smc6 complex. A DSB was induced at the MAT locus in wildtype (CCG6983) and smc6–9 (CCG6985) strains expressing Ddc2-GFP and Rtt107-9myc tagged at non-permissive (37°C) temperature. The binding of Rtt107 around the MAT DSB locus on chromosome III was assayed by chromatin immunoprecipitation at the indicated time points, after (cut- 2 hours) and before (uncut) DSB induction in wildtype and smc6–9 cells at 37°C. The averages of two independent experiments with corresponding standard deviations are shown. A locus on chromosome VI was used as a control.