. 2011 May 25;6(5):e20212. doi: 10.1371/journal.pone.0020212

Table 1. Correlations of in vitro IC's values between the different drugs tested in 46 field isolates as well as associations between the IC's values and the major polymorphisms in pfmdr1 and pfmrp1 genes.

	In vitro drug IC's significance^§							pfmdr1 polymorphisms significance^¤						pfmrp1 polymorphisms significance^¤
	ART IC₉₀	DHA IC₅₀	DHA IC₉₀	MQ IC₅₀	MQ IC₉₀	LUM IC₅₀	LUM IC₉₀	130 E-K	184 Y-F	249 syn	750 A-T	1226 F-Y	CNV	325 N-S	572 F-L	785 H-N	876 I-V	1007 T-M	1390 F-I
ART IC₅₀	<0.001 (0.97)	<0.001 (0.68)	<0.001 (0.66)	<0.001 (0.82)	<0.001 (0.69)	<0.001 (0.88)	<0.001 (0.80)	0.855	0.155	0.855	0.528	0.003	<0.001	0.628	0.141	0.181	0.791	0.181	0.007
ART IC₉₀		<0.001 (0.6)	<0.001 (0.63)	<0.001 (0.82)	<0.001 (0.73)	<0.001 (0.81)	<0.001 (0.77)	0.942	0.353	0.942	0.626	0.005	<0.001	0.533	0.120	0.145	0.623	0.145	0.004
DHA IC₅₀			<0.001 (0.90)	0.001 (0.49)	0.027 (0.33)	<0.001 (0.70)	<0.001 (0.54)	0.395	0.467	0.395	0.596	0.354	0.284	0.925	0.744	0.416	0.926	0.416	0.163
DHA IC₉₀				0.004 (0.41)	0.075 (0.27)	<0.001 (0.63)	<0.001 (0.51)	0.974	0.538	0.974	0.870	0.292	0.152	0.925	0.566	0.398	0.922	0.398	0.192
MQ IC₅₀					<0.001 (0.96)	<0.001 (0.81)	<0.001 (0.80)	0.606	0.249	0.606	0.342	0.004	<0.001	0.835	0.116	0.174	0.722	0.174	0.003
MQ IC₉₀						<0.001 (0.66)	<0.001 (0.72)	0.458	0.389	0.458	0.233	0.004	<0.001	0.886	0.063	0.113	0.976	0.113	0.004
LUM IC₅₀							<0.001 (0.90)	0.527	0.163	0.527	0.320	0.004	<0.001	0.755	0.238	0.209	0.801	0.209	0.015
LUM IC₉₀								0.181	0.249	0.181	0.087	0.001	<0.001	0.526	0.124	0.224	0.903	0.224	0.022

^§

Cells show P-value (2-tailed) and in brackets the Pearson correlation coefficient between the IC's values of the different antimalarial tested in the isolates. P value<0.05 is considered significant (2-tailed).

^¤

Cells show P-value (2-tailed) of the linear association analysis between the main polymorphisms found in the isolates (Table 2) and the in vitro drug IC's values. Isolates that harbors both alleles (mixed infection) in the determined polymorphic region were excluded from the statistical analysis. T-test with equal variances assumed was used to test differences. Bold numbers are considered significant for T-test (P<0.05). For comparison, Bonferroni-adjusted levels of significance would be: P value<0.0042.

ART: artemisinin, DHA: dihydroartemisinin, MQ: mefloquine, LUM: lumefantrine, IC₅₀ and IC₉₀: Inhibition Concentration of 50% and 90%. CNV: copy number variation.