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. 2011 May 25;6(5):e20173. doi: 10.1371/journal.pone.0020173

Figure 5. Impaired OL development in Hx−/− mice.

Figure 5

Brain sections of wild-type and Hx−/− mice were immunoreacted to discriminate between OPCs and mature OLs and OPCs and OLs counted as reported in Materials and Methods. A) Quantification of PDGFRα-positive cells demonstrated similar numbers of OPCs in both cerebral cortex and corpus callosum in Hx−/− and wild-type mice at P10. On the contrary, the number of CC1-positive, GFAP-negative mature OLs in Hx−/− mice was strongly reduced compared to wild-type animals at P10 and P20. Data represent mean ± SEM, n = 3 mice for each genotype. **  = P<0.01, ***  = P<0.001. B) Maps, obtained with Neurolucida/Neuroexplorer, of brain sections of PDGFRα- (left) and CC1- (right) positive cells, respectively, in Hx−/− and wild-type mice at P10. Red  =  OPCs, blue  =  mature OLs. Note the reduced number of mature OLs in the supragranular layer of cortex in Hx−/− mice. C) Representative pictures of CC1/GFAP double staining for CC1 (brown) and GFAP (grey) in brain sections of a wild-type and a Hx−/− mouse. The latter shows a strong reduction in the number of CC1 positive cells in the cortex (arrows) and in corpus callosum, CC, (arrow-heads) compared to wild-type animal. Bar  = 50 µm.