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. 2011 May 25;6(5):e20514. doi: 10.1371/journal.pone.0020514

Figure 2. Site-specific targeting without sequence homology.

Figure 2

A. DR-GFP can be integrated at the p84 locus in either orientation. B. PCR with p84-5′ and GFP-3′ primers demonstrates three 293T clones with site-specific integration. λ indicated DNA ladder. C. FISH demonstrates colocalization of probes against DR-GFP (green) and spanning the p84 locus (orange) in 293T cells. D. Sequences at 5′ and 3′ borders of integrated DR-GFP. Red sequence is from the p84 locus and blue sequence is from the DR-GFP construct. Integration with no sequence modification is indicated as ‘unmod’.