Table 1. PCR and qPCR primers.

Amplicon		Primers (Forward then Reverse)
I-SceI or LweI site-loss		5′- AGGGCGGGGTTCGGCTTCTGG 5′- CCTTCGGGCATGGCGGACTTGA
Reporter copy number for quantitative PCR		5′- CGGCGCCGGCAGGAAGGAA 5′- CTCTAGAGCCGCCGGTCACACG
Reference amplicon from APE1 on chr.14 for quantitative PCR		5′- CGGCACGCGTGGGATGAA 5′- GCCTTGGCGCTCTTGTGG
Nested PCR to determine I-SceI cleavage efficiency	1st round, PCR ×18 cycles	5′- AGGGCGGGGTTCGGCTTCTGG 5′- CCTTCGGGCATGGCGGACTTGA
	2nd round, quantitative PCR	5′- GACGTAAACGGCCACAAGTT 5′- AAGTCGTGCTGCTTCATGTGor5′- GCAACGTGCTGGTTATTGTG 5′- TGCCTCGTGGGTCTTCTACT
Reference amplicon from near p84 on chr.19 for PCR of I-SceI cleavage efficiency	1st round, PCR ×18 cycles	5′- TTCTGTGCTGGGGTAGAACC 5′- CCAAACCCCAGTCCTCTACA
	2nd round, quantitative PCR	5′- CTCAAACTGCATGGCTCAAA 5′- CCAAACCCCAGTCCTCTACA
Confirming HPRT-DRGFP targeting at p84 without homologous arms	Same orientation as p84 coding	5′ junction	5′-AGGATCCTCTCTGGCTCCAT 5′-CGAGATCTGATGCCCTCTTC
		3′ junction	5′-TGTAGAGGACTGGGGTTTGG 5′-CTTGCTTTCTTTGCCTGGAC
	Opposite orientation	5′ junction	5′-CAGCTCAGGTTCTGGGAGAG 5′-TGTAGAGGACTGGGGTTTGG
		3′ junction	5′-GCAAACATGCTGTCCTGAAG 5′-TTGCTCTCTGCTGTGTTGCT