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. 2011 May 25;6(5):e20439. doi: 10.1371/journal.pone.0020439

Figure 2. Recombinant expression and purification of Cr-DapL from E. coli.

Figure 2

Lane (1)–Protein marker (kDa), Lane (2)–10 µg uninduced soluble protein extract, Lane (3)–10 µg induced soluble extract, Lane (4)–10 µg of post binding protein, Lane (5)–1.0 µg pure recombinant Cr-DapL protein. The proteins were resolved on a SDS-PAGE gel containing 10% (w/v) acrylamide and the gel was stained using Coomassie Blue.