Figure 2. Recombinant expression and purification of Cr-DapL from E. coli.
Lane (1)–Protein marker (kDa), Lane (2)–10 µg uninduced soluble protein extract, Lane (3)–10 µg induced soluble extract, Lane (4)–10 µg of post binding protein, Lane (5)–1.0 µg pure recombinant Cr-DapL protein. The proteins were resolved on a SDS-PAGE gel containing 10% (w/v) acrylamide and the gel was stained using Coomassie Blue.