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. 2011 May 25;6(5):e20439. doi: 10.1371/journal.pone.0020439

Figure 3. Functional complementation of the E. coli dapD/E mutant.

Figure 3

Functional complementation was tested using the E. coli dapD/E double mutant (AOH1). The plasmids pBAD33 and pBAD33+Cr-DapL were selected on LB agar medium supplemented with 50 µg mL−1 DAP and 34 µg mL−1 chloramphenicol. The bacteria were grown to an OD of 0.5 at 600 nm, the strain were serially diluted to 10−1, 10−2, 10−3 and 10−4 using 0.85% (w/v). The strain harboring the pBAD33 and pBAD33+Cr-DapL was replica-plated onto LB medium plus 0.2% (w/v) arabinose with or without 50 µg mL−1 DAP. The cultures were grown at 30 °C for 24 hours.