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. 2011 May 25;6(5):e20439. doi: 10.1371/journal.pone.0020439

Table 1. Substrate specificity of Cr-DapL using four amino donors.

Amino Donor Relative Activity (%) Amino Acceptor Relative Activity (%)
l,l-DAP 100 2-Ketoglutarate 100
meso-DAP 0 Pyruvate 0
l-Lysine 0 Prephenate 0
l-Ornithine 0 Oxaloacetate 0
Oxovelarate 0

Relative substrate specificity of Cr-DapL using various amino donors. The assay measured the production of dihyrdoquinazolium using the OAB assay at 400 nm using 0.5 mM amino donor and 2 mM 2-oxoglutarate. Relative substrate specificity of Cr-DapL using various amino acceptors. The assay measured the production of dihyrdoquinazolium using the OAB assay at 400 nm using 2 mM of each acceptor and 0.5 mM of l,l-DAP.