Table 1. Substrate specificity of Cr-DapL using four amino donors.
Amino Donor | Relative Activity (%) | Amino Acceptor | Relative Activity (%) |
l,l-DAP | 100 | 2-Ketoglutarate | 100 |
meso-DAP | 0 | Pyruvate | 0 |
l-Lysine | 0 | Prephenate | 0 |
l-Ornithine | 0 | Oxaloacetate | 0 |
Oxovelarate | 0 |
Relative substrate specificity of Cr-DapL using various amino donors. The assay measured the production of dihyrdoquinazolium using the OAB assay at 400 nm using 0.5 mM amino donor and 2 mM 2-oxoglutarate. Relative substrate specificity of Cr-DapL using various amino acceptors. The assay measured the production of dihyrdoquinazolium using the OAB assay at 400 nm using 2 mM of each acceptor and 0.5 mM of l,l-DAP.