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A) Proportional Venn diagram illustrating the number of isolated peptides and percentage coverage of bacteriorhodopsin digestion using EFO-IMER or in-solution digestion by LC-MS/MS analysis and MASCOT database searches. B) Representative chromatograms of in-solution-digested bacteriorhodopsin (upper panel) or EFO-IMER-digested bacteriorhodopsin (lower panel) indicating the considerably greater peptide extraction from the hydrophobic protein.
