Fig. 1. Immunofluorescence staining of E.coli J96 cells. Bacteria grown in LB at 37°C were prepared for microscopic analysis as described in Materials and methods. (A) Phase-contrast micrograph of bacterial cells. (B) Anti-Pap–lissamine–rhodamine staining. (C) Anti-type-1–fluorescein isothiocyanate staining.