Fig. 3. Effect of purified His-PapB on the in vitro recombination in bacterial extracts containing either FimB or FimE. Recombination assays were set up as described in Materials and methods. Lane 1: control extract (pET11 in NEC26) plus pMM36 (switch starts in the on orientation). Lane 2: FimE extract (pIB382 in NEC26) plus pMM36. Lane 3: FimE extract with 4 μM His-PapB plus pMM36. Lane 4: FimE extract with 8 μM His-PapB plus pMM36. Lane 5: FimE extract with 12 μM His-PapB plus pMM36. Lane 6: FimE extract with 16 μM His-PapB plus pMM36. Lane 7: 1 kb marker. Lane 8: FimB extract (pIB378 in NEC26) plus pMM36. Lane 9: FimB extract with 4 μM His-PapB plus pMM36. Lane 10: FimB extract with 8 μM His-PapB plus pMM36. Lane 11: FimB extract with 12 μM His-PapB plus pMM36. Lane 12: FimB extract with 16 μM His-PapB plus pMM36. Lane 13: control extract (pET11 in NEC26) plus pJL2 (switch in the off orientation). Lane 14: FimB extract plus pJL2. Lane 15: FimB extract with 4 μM His-PapB plus pJL2. Lane 16: FimB extract with 8 μM His-PapB plus pJL2. Lane 17: FimB extract with 12 μM His-PapB plus pJL2. Lane 18: FimB extract with 16 μM His-PapB plus pJL2.