Table I. Inhibition of type 1 fimbriae production by PapB.
Strain | Plasmid | HA phenotypea |
HA titreb | EMc % piliated bacteria | |
---|---|---|---|---|---|
–mannose | +mannose | ||||
HB101 | – | – | nd | 0 | |
HB101 | pSH2 | +++ | – | 1/128 | 77 |
HB101 | pSH2 + pBR322 | ++ | – | 1/128 | 78 |
HB101 | pSH2 + pHMG80 | – | – | 1/16 | 2 |
HB101 | pSH2 + pHMG95 | ++ | – | 1/128 | 82 |
aThe haemagglutination (HA) test was performed with human erythrocytes as described in Materials and methods.
bThe HA titre was determined as the highest dilution of the bacterial culture with which the haemagglutination with guinea pig erythrocytes could still be clearly observed (nd, not determined).
cThe EM test was performed as described in Materials and methods.