. 2000 Apr 3;19(7):1450–1457. doi: 10.1093/emboj/19.7.1450

Table IV. Strains and plasmids used in this study.

Strain/plasmid	Genotype/phenotype	Reference/source
E.coli strain
HB101	F^– lacY1 recA13	Boyer and Roulland-Dussoix (1969)
JM109	F′ lacI^q recA1	Yanisch-Perron et al. (1985)
AAEC198A	MG1655 ΔlacZYA fimA–lacZYA (switch locked on)	Blomfield et al. (1991, 1993)
AAEC370A	MG1655 ΔlacZYA fimA–lacZYA fimE-am18 (switch starts on)	Blomfield et al. (1993)
AAEC374A	MG1655 ΔlacZYA fimA–lacZYA fimB-am6 fimE-am18	Blomfield et al. (1993)
BGEC056	MG1655 fimB–lacZYA FimB–LacZ	I.C.Blomfield (unpublished)
BGEC088	MG1655 Δlac fimE–lacYA FimE–LacZ	I.C.Blomfield (unpublished)
NEC026	BL21 (DE3) Δfim	Gally et al. (1996)
Plasmid
pQE30	expression vector, Cb^r	Qiagen manufacturer's instruction
pYN1	wt papB gene cloned into pQE30, Cb^r	Xia et al. (1998)
pSH2	pACYC184 J96 fim B-H, Cm^r	Orndorff and Falkow (1984)
pBR322	cloning vector, Cb^r Tc^r	Bolivar et al. (1978)
pHMG80	lacP(UV5) papB⁺/pBR322	this laboratory (unpublished)
pHMG95	lacP(UV5) papI⁺/pBR322	this laboratory (unpublished)
pACYC184	low-copy-number cloning vector, Tc^r Cm^r	Chang and Cohen (1978)
pHMG98	lacI^Q lacP(UV5) papI⁺ /pACYC184	Forsman et al. (1989)
pHMG88	lacI^Q lacP(UV5) papB⁺ /pACYC184	Forsman et al. (1989)
pRHU845	pap gene cluster cloned in pACYC184	Norgren et al. (1984)
pET11	T7 promoter expression vector, Cb^r	Linn and St. Pierre (1990)
pIB378	pET11, fimB	Gally et al. (1996)
pIB382	pET11, fimE	Gally et al. (1996)
pMM36	fim cloned between transcriptional terminator rrnBT1T2 and T4 gene 32 terminator, Cm^r (switch on)	McClain et al. (1991)
pJL2	fim cloned between transcriptional terminator rrnBT1T2 and T4 gene 32 terminator, Cm^r (switch off)	McClain et al. (1991)