Table 3.

Predicted N-glycosylation sites for vertebrate lipoprotein lipases

Vertebrate	Species	Site 1	Site 2	Site 3	Site 4	Site 5	Site 6	Site 7	No of Sites
Human	Homo sapiens		70NHS		386NKT				2
Chimpanzee	Pan troglodytes		70NHS		386NKT				2
Orangutan	Pongo abelii		70NHS		386NKT				2
Rhesus	Macaca mulatta		70NHS		386NKT				2
Baboon	Papio anubis		70NHS		386NKT				2
Marmoset	Callithrix jacchus		70NHS		386NKT				2
Mouse	Mus musculus		70NHS		386NKT				2
Rat	Rattus norvegicus		70NHS		386NKT	411NDS			3
Guinea Pig	Cavia porcellus		70NHS		386NNT				2
Horse	Equus caballus		63NQS		379NKT				2
Cow	Bos taurus		73NHS		389NKT				2
Dog	Canis familaris		66NHT		382NKT				2
Rabbit	Oryctolagus cuniculus		69NHS		385NKT			471NKS	3
Pig	Sus scrofa		73NHS		389NKT				2
Elephant	Loxodonta africana		70NYS		386NKT				2
Opossum	Monodelphis domestica		73NHS		389NKT		452NIS		3
Platypus	Ornithorhynchus anatinus		71NHT		387NKT				2
Chicken	Gallus gallus		70NHT	354NVT	386NKT				3
Frog	Xenopus tropicalis		60NHT	344NLT	376NKT				3
Stickleback	Gasterosteus aculeatus	34NTT			407NTT				2

Numbers refer to amino acids in the acid sequences, including N-asparagine; K-lysine; I-isoleucine; H-histidine; S-serine; T-threonine; Q-glutamine; D-aspartate; Y-tyrosine; and V-valine. Note that there are 7 potential sites identified, including 2 sites for human LPL (sites 2 and 4) (see Kobayashi et al, 1994; van Tilbeurgh et al, 1994). N-glycosylation sites were identified using the NetNGlyc 1.0 web server (http://www.cbs.dtu.dk/services/NetNGlyc/).