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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Vasc Surg. 2011 Apr 22;53(6):1565–1574.e1. doi: 10.1016/j.jvs.2011.01.074

Figure 1.

Figure 1

Bone marrow aspirate is loaded into a closed sterile 60-mL MarrowStim™ centrifugation tube in the operating room (A) and placed into the centrifuge device where the buffy coat is separated at 1500 rpm for 15 min (B). The ABMNC rich buffy coat is aspirated from the tube (C) and and 0.75 ml. aliquots are delivered via IM injections at 2-cm intervals, 1.5-cm deep into the gastrocnemius muscle, along the medial and lateral aspect of the index limb (D).