. 2000 Apr 3;19(7):1467–1475. doi: 10.1093/emboj/19.7.1467

Table I. Interactions detected by the yeast two-hybrid system.

Binding domain fusion^a	Activation domain ^fusion	β-Galactosidase activity^b
^(A)
^{SpoIIE (II–III)}	^None	^–
^None	^{SpoIIE (II–III)}	^–
^{SpoIIE (II)}	^None	^–
^None	^{SpoIIE (II)}	^–
^{SpoIIE (III)}	^None	^–
^None	^{SpoIIE (III)}	^–
^{SpoIIE (II–III)}	^{SpoIIE (II–III)}	⁺⁺
^{SpoIIE (II)}	^{SpoIIE (II)}	^–
^{SpoIIE (III)}	^{SpoIIE (III)}	^–
^{SpoIIE (II–III)}	^{SpoIIE (III)}	^–
^{SpoIIE (III)}	^{SpoIIE (II–III)}	^–
^{SpoIIE (II)}	^{SpoIIE (II–III)}	^–
^{SpoIIE (II–III)}	^{SpoIIE (II)}	^–
^(B)
^None	^FtsZ	^–
^{SpoIIE (II–III)}	^FtsZ	⁺
^{SpoIIE (II)}	^FtsZ	^–
^{SpoIIE (III)}	^FtsZ	^–

^aSpoIIE (II–III), SpoIIE (II) and SpoIIE (III) contain residues 326–827, 326–567 and 568–827, respectively.

^bDetected by colour development using X-gal. ++, detectable colour development within 2 h; +, detectable colour development within 3 h; –, no significant colour development after 8 h.