Table II. Strains and plasmids.
| Strain/plasmid | Relevant genotypea | Construction, source or reference |
|---|---|---|
| B.subtilis | ||
| SG38 | trpC2 amyE | Errington and Mandelstam (1986) |
| E.coli | ||
| DH5α | F– endA1 hsdR17 supE44 λ– thi-1 recA1 gyrA96 relA1 Δ(lacZYA-argF) U169 φ80dlac Δ(lacZ)M15 | Gibco-BRL |
| C41 (DE3) | BL21 (DE3) derivative | Miroux et al. (1996) |
| S.cerevisiae | ||
| Y187 | MATα ura3-52 his3-200 ade 2-101 trp 1-901 leu 2-3 112 gal4Δ met– gal80Δ URA3::GAL1UAS-Gal1TATA-lacZ | Clontech |
| Plasmids | ||
| pAS2-1 | GAL4 DNA-binding domain vector | Clontech |
| pACT2 | GAL4 activation domain vector | Clontech |
| pSG1711 | pACT2 containing ftsZ | A.Marston (unpublished) |
| pSG1904 | pAS2-1 containing spoIIE(976–2481) | this work |
| pSG1905 | pACT2 containing spoIIE(976–2481) | this work |
| pSG1906 | pAS2-1 containing spoIIE(1702–2481) | this work |
| pSG1907 | pACT2 containing spoIIE(1702–2481 | this work |
| pSG1916 | pAS2-1 containing spoIIE(976–1701) | this work |
| pSG1917 | pACT2 containing spoIIE(976–1701) | this work |
| pET-11a | T7 expression vector | Novagen |
| pET-21d | T7 expression vector | Novagen |
| pTYB3 | expression vector for Intein-chitin binding domain fusion protein | New England BioLabs |
| pRB1011 | pET-11a containing spoIIE | Lucet et al. (1999) |
| pIL1 | pET-11a containing strep′-′spoIIE | this work |
| pSG1909 | pET-21d containing strep′-′spoIIE(961–2481) | this work |
| pSG1911 | spoIIE(961–2481)′-′VMA intein′-′chitin-binding domain | this work |
| pSG4600 | pET-3a containing strep′-′mbl | R.Carballido-López (unpublished) |
aNumbers in parentheses after spoIIE refer to the first and last nucleotides of the insert from the spoIIE coding sequence.