Table I. Phosphatidylinositols released from purified T.cruzi GPI-anchored glycoconjugates by nitrous deamination.
| Pseudomolecular iona [M-H]– m/z | Octyl-Sepharose fraction (see Figure 1A and B) |
Lipid component of the PI moiety | ||||
|---|---|---|---|---|---|---|
| Trypomastigote (%) |
Epimastigote (%) |
|||||
| I | II | I | II | III | ||
| 557 | 95 | 11 | 29 | 13 | 2 | 1-O-(C16:0)lyso-alkylglycerol |
| 571 | trb | 0 | 17 | 5 | 7 | (C16:0)lyso-acylglycerol |
| 599 | tr | 0 | 12 | tr | tr | (C18:0)lyso-acylglycerol |
| 627 | tr | 0 | 12 | 0 | 0 | (C20:0)lyso-acylglycerol |
| 655 | tr | 0 | 6 | 0 | 0 | (C22:0)lyso-acylglycerol |
| 778 | 0 | 0 | 0 | 0 | 6 | ceramide, (C16:0)fatty acid-(C18:1)sphingosine |
| 780 | 0 | 0 | 0 | 0 | 8 | ceramide, (C16:0)fatty acid-(C18:0)sphinganine |
| 795 | 5 | 37 | 24 | 77 | 16 | 1-O-(C16:0)alkyl-2-O-(C16:0)acylglycerol |
| 819 | tr | 21 | 0 | 0 | 0 | 1-O-(C16:0)alkyl-2-O-(C18:2)acylglycerol |
| 821 | 0 | 31 | 0 | 0 | 0 | 1-O-(C16:0)alkyl-2-O-(C18:1)acylglycerol |
| 823 | 0 | 0 | 0 | 2 | 0 | 1-O-(C16:0)alkyl-2-O-(C18:0)acylglycerol |
| 890 | 0 | 0 | 0 | 0 | 18 | ceramide, (C24:0)fatty acid-(C18:1)sphingosine |
| 892 | 0 | 0 | 0 | 3 | 43 | ceramide, (C24:0)fatty acid-(C18:0)sphinganine |
aOther minor pseudomolecular ion species that could not be characterized by ES-MS–CID-MS as inositolphospholipids were not included in this table. btr = trace.