Figure 1.

CCA1-LUC and TOC1-LUC Degradation Rates under Different Light Regimes

(A) Degradation rates of CCA1-LUC (blue traces) and TOC1-LUC (red traces) calculated from the curve fitting to the exponential phase of decay following inhibition of de novo protein synthesis with cycloheximide. The x axis indicates treatment time; light regime is indicated in the panels. Error bars represent standard error of the mean (SEM; n = 5). Decay rates measured for free luciferase ranged from 0.165 to 0.136 hr⁻¹, as indicated by the horizontal dotted lines.

(B) Number of CCA1-LUC (blue line) or TOC1-LUC (red line) molecules/cell for an LD12:12 cycle calculated by in vitro luciferase activity of cell extracts (mean values plotted ± SEM; n = 2).

(C) Absolute degradation rates in molecules/cell/hr for CCA1-LUC (blue lines) and TOC1-LUC (red lines) obtained from multiplying decay rates by molecule number (mean values plotted ± SEM; n = 2). See also Figure S1.