Figure 4.

Application of MG132 Arrests Cytosolic Oscillations

(A) Three independent 48 hr time series of protein extracts in constant light with vehicle or MG132 treatment starting after 12 hr. Samples were run on immunoblots using a PRX-SO_2/3 antibody. For equal loading control, Coomassie staining of Rubisco (RbcL) is shown on a representative gel (bottom panels).

(B) Densitometry performed with ImageJ64 showing grouped data of the three replicates for vehicle-treated (black line) or MG132-treated (blue line) cells. Dotted lines indicate SD; blue shaded area indicates window of inhibition. See also Figure S4.