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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Radiat Res. 2011 Jan 25;175(4):493–500. doi: 10.1667/RR2431.1

FIG. 1.

FIG. 1

Genotyping of the LEWES strain. The R2140C SNP located downstream of the leucine zipper abolishes a BsmB1 site while the M3844V SNP in the kinase domain creates a novel Hph1 site. PCR products from both SNP loci were purified and digested with BsmB1 or Hph1. Both C57BL/6 and LEWES mouse products were cleaved with BsmB1, while the BALB/c mouse product containing R2140C was not (arrow). After Hph1 digestion only one cut occurred in the C57BL/6 mouse products, but BALB/c and LEWES mouse products containing the M3844V SNP yielded an extra digested fragment (arrows).