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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Crit Care Med. 2011 Jun;39(6):1423–1430. doi: 10.1097/CCM.0b013e318212020a

Figure 5. Hippocampal Histology after Post-Cardiac Arrest Therapeutic Hypothermia.

Figure 5

Representative hippocampal sections from sham injured rats or rats 7 days after 10-minute asphyxial cardiac arrest followed by normothermia or TH initiated 1 hour after ROSC and maintained for 24 or 48 hours. A. Sections immunolabeled for NeuN (red) and counterstained with Hoechst (blue). Degeneration of CA1 pyramidal neurons is characterized by loss of NeuN immunolabeling (red) and condensed shrunken nuclei (blue). B. Sections stained by the H&E method. Classic ischemic neuronal change in the CA1 pyramidal layer is characterized by shrunken dark-staining pyknotic nuclei (blue) and eosinophilic cytoplasm (pink) see in the high power images. Scale bar 500μm for low power images and 50μm for high power images.