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. Author manuscript; available in PMC: 2012 May 27.
Published in final edited form as: Chem Biol. 2011 May 27;18(5):589–600. doi: 10.1016/j.chembiol.2011.03.010

Figure 2. Effect of thiostrepton and micrococcin on GTPase activity of various translation factors.

Figure 2

(A–F) The rdGTPase activities of translation factors (A) E. coli EF-G, (B) TetM, (C) E. coli EF4, (D) E. coli BipA, (E) E. coli IF2 and (F) E. coli EF-GΔG′, using E. coli 70S ribosomes in the absence (black circles) and presence of 1 μM thiostrepton (ThS, red squares) or 5 μM micrococcin (MiC, blue triangles). (G–I) The rdGTPase activities of E. coli translation factors (G) EF-G, (H) EF4 and (I) BipA, using E. coli 70S ribosomes lacking L7/L12. Reactions in (G–I) were incubated for 12 hours at 20 °C. In all cases, background hydrolysis due to the intrinsic GTPase activity of each factor has been subtracted. See also Figure S2.