Table 1. Summary of murine xenotransplantation results.
Animals 1–4 were injected with mock-infected HPCs and successfully engrafted. Animals 5–11 were injected with virus-treated HPCs and were successfully engrafted with human cells. Animals 12–17 were injected with virus-treated HPCs but did not successfully engraft. Percents of human and GFP+ cells are percents of total peripheral blood leukocytes at 14–18 weeks after transplant.
Animal | Number of cells transplanted |
Condition | % Human Leukocytes |
% of Human Leukocytes that are GFP+ |
% of GFP+ Human Leukocytes that are: |
||
---|---|---|---|---|---|---|---|
Myeloid | B cells | T cells | |||||
1 | 50,000 | Mock | 0.6 | 0.0 | - | - | - |
2 | 50,000 | Mock | 0.1 | 0.0 | - | - | - |
3 | 100,000 | Mock | 0.1 | 0.0 | - | - | - |
4 | 150,000 | Mock | 0.1 | 0.0 | - | - | - |
5 | 50,000 | 7SF-GFP/HXB | 0.1 | 98 | 16 | 78 | 0.0 |
6 | 50,000 | 7SF-GFP/HXB | 1.4 | 100 | 4.9 | 92 | 0.1* |
7 | 100,000 | 7SF-GFP/HXB | 0.4 | 99 | 19 | 56 | 0.0 |
8 | 150,000 | 7SF-GFP/HXB | 9.6 | 16 | 6.9 | 88 | 0.0 |
9 | 150,000 | 7SF-GFP/HXB | 0.5 | 46 | 7.7 | 77 | 0.0** |
10 | 100,000 | 7SF-GFP/HXB | 1.4 | 0.0 | - | - | - |
11 | 100,000 | 7SF-GFP/HXB | 0.6 | 0.0 | - | - | - |
12–17 | 150,000 | 7SF-GFP/HXB (No engraftment) | 0.0 – 0.03 | 0.0 | - | - | - |