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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Food Chem. 2011 Sep 15;128(2):513–519. doi: 10.1016/j.foodchem.2011.03.073

Figure 1. Kaempferol inhibits proliferation of ovarian cancer cells at 40-uM or higher concentrations.

Figure 1

A. OVCAR-3, A2780/wt, and A2780/CP70 cells were seeded into 96-well plates at 2000 cells/well and incubated overnight before treatment with various concentration of kaempferol in triplicate for 24 hours. After removing medium, the plate was frozen at -20°C for 30 minutes, thawed at room temperature, and cells were lysed with 200 μl 1x CyQUANT cell lysis buffer containing 5X SYBR Green I dye. After 5 minutes incubation at room temperature, cell lysates (50 μl) were transferred to PCR strip tubes and the fluorescence were measured at 90 °C in a real-time PCR instrument. Data represent Means ± SE from 3 independent experiments. * p<0.05, **p<0.01 as compared to control. B. A standard curve was generated by seeding different numbers of OVCAR-3 cells in 96-well plate, incubating them overnight, and measuring DNA abundance as described above in A.