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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Food Chem. 2011 Sep 15;128(2):513–519. doi: 10.1016/j.foodchem.2011.03.073

Figure 2. Kaempferol has no cytotoxicity on ovarian cancer cells.

Figure 2

Ovarian cancer cells were seeded in 96-well plate at 5000 cells/well and incubated overnight before treatment with kaempferol for 24 hours. Culture medium was sampled for measurement of free LDH levels. Cell culture were then added with Lysis Solution and incubated at 37°C for 1 hour before being sampled again to measure total LDH levels. LDH levels were analyzed with a CytoTox 96 Non-Radioactive Cytotoxicity Assay from Promega. None-cell culture medium was assayed to the control background, and contained LDH levels were derived by subtracting free LDH levels from total LDH levels. All LDH levels within a single experiment were standardized to the total LDH level of control for statistical analysis. Data represent Mean ± SE from 2 or 3 independent experiments.